FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l04n01_151022_gms3.3510000003a522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l04n01_151022_gms3.3510000003a522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35111978
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACAACAAAGGCTTAATCTCAGCAGATCGGAAGAGCACACGTCTGAA1266460.36069172747829814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT174900.035.0725252
TCATAGG176800.034.3196712
GGGAGAC214600.033.9522361
CGACGTA182450.033.4218943
CATAGGA182850.033.23334513
ATAGGAT185550.032.7014414
GCTGTAA77700.032.4920773
GGATCAT197400.030.72753317
CTGTAAT83500.030.610014
TAGGATC198750.030.60937715
TTCATAG201750.030.13118411
GGCTGTA85050.029.976892
CTATAGG107650.029.42574526
TAATCTC244800.029.00550818
ACTTCAT215050.028.6344229
AGGCTTA252900.028.41387613
CTTCATA215950.028.3379710
CAAAGGC258300.028.03816810
TTCTATA185450.027.56260924
GTAATGG92750.027.3617156
Produced by FastQC (version 0.11.2)