FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l04n01_151022_gms2.3510000003a515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l04n01_151022_gms2.3510000003a515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35221638
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC5351821.5194693670975778TruSeq Adapter, Index 9 (100% over 50bp)
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAAGATCGGAAG378780.10754184686129588No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG376000.10675255932163064TruSeq Adapter, Index 9 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG170250.037.8677941
TCGACGT173400.037.077372
GACGTAC173000.037.0262374
CGTATGC675800.036.92452245
TCATAGG172250.036.91079712
GCTGTAA98550.035.6643183
CATAGGA182700.034.811913
CTGTAAT103400.034.0752834
ACGTACT189700.033.978145
CGTACTT190100.033.9066476
ATAGGAT188350.033.76806614
CGACGTA190850.033.7432563
GGATCAT188900.033.5867617
CGGCTCG12250.033.42514426
CGATATA117100.033.0123023
TGTAATG109300.032.2133985
TATAGTG129100.031.8386526
GGCTGTA111200.031.8123342
TAGTGTA174200.031.7968948
GTAGTGT39250.031.755027
Produced by FastQC (version 0.11.2)