FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l04n01_151022_gms1.3510000003a4fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l04n01_151022_gms1.3510000003a4fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44039229
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC813760.18478070994385482TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGG81900.029.58503712
GACGTAC82300.029.52554
AACGGCT135750.028.58854314
TAACGGC136250.028.43393113
CATAGGA86650.028.01516513
TATAGTG115200.026.9510846
CGGCTCG11250.026.59723326
TAGTGTA155350.026.3145718
ACGGCTA150400.025.8489315
CGATATA116650.025.8287663
GCTGTAA96300.025.8192623
CGACGTA98450.024.981143
CATCACG159900.024.2563925
CTGTAAT103300.024.11134
ACGTACT102300.024.037775
TGTAACG163900.023.8979211
ATAGGAT101200.023.8318114
CTATCAC166400.023.62037519
ATCACGC125900.023.55195626
TCGACGT106050.023.5103782
Produced by FastQC (version 0.11.2)