FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_60.3510000003840f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_60.3510000003840f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14082040
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC10379407.370665045689403TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1151100.044.33466312
ACACGTC1151000.044.2818313
AGAGCAC1160750.044.165728
ACGTCTG1155150.044.11051615
GAGCACA1159750.044.108759
TCGGAAG1167300.044.0760573
AGCACAC1158650.044.0690710
CGTCTGA1156050.044.01615516
TCTGAAC1152150.043.96822418
GCACACG1162350.043.95395311
CTGAACT1154350.043.8201119
AGTCACT1146000.043.617128
GTATGCC1143050.043.59694745
GTCACTA1140950.043.58991629
ACTAGCT1136400.043.55569532
CACTAGC1136250.043.5416431
TAGCTTA1132750.043.5000134
AGCTTAT1134850.043.42028435
GCTTATC1138850.043.41398636
TTATCTC1147300.043.40607538
Produced by FastQC (version 0.11.2)