FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_5.351000000383a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_5.351000000383a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16674753
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC3335632.0004074423171367TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG389450.043.00032815
ACACGTC390300.042.95048513
TCTGAAC388550.042.91643518
CGTCTGA390250.042.84325416
CACACGT391200.042.82866712
GTCACAC383100.042.54203829
CACACTT379000.042.5369931
TCACACT379500.042.52838530
CTGAACT394100.042.24353819
AGAGCAC398100.042.148518
AGCACAC399700.041.95164510
GTATGCC383050.041.94564445
GAGCACA401000.041.871759
TCGGAAG405200.041.6124843
AATCTCG384750.041.48781639
TCTCGTA387900.041.4351541
GCACACG405800.041.37647211
CGTATGC389800.041.23083544
ACACTTG399100.040.3439532
ATCTCGT397550.040.32748840
Produced by FastQC (version 0.11.2)