FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_300.35100000038429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_300.35100000038429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14054275
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2658251.8914173801209953TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA318050.042.75028216
CACACGT319450.042.7440812
ACACGTC319700.042.68250713
ACGTCTG319000.042.65103515
TCTGAAC319000.042.42608618
GCTACAT304650.042.3566735
GTATGCC312250.042.21850645
TCTCGTA313850.042.07631341
CGTATGC314250.041.96413444
AGAGCAC325850.041.952888
CTGAACT322600.041.91776719
AGCACAC327300.041.78077310
GAGCACA327650.041.7704739
CTACATC309500.041.7147336
GCACACG329800.041.4913511
CACGGCT318550.041.249231
ATCTCGT318850.041.12717440
TCGGAAG334650.040.9467163
AGTCACG326600.040.56144328
CATCTCG325650.040.0680139
Produced by FastQC (version 0.11.2)