FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_30.351000000383e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_30.351000000383e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685656
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC6121054.168046698084171TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT698700.043.7540812
ACACGTC697700.043.713613
AGAGCAC705250.043.5423138
ACGTCTG698500.043.5205215
CGTCTGA698350.043.50086616
GAGCACA707100.043.3711179
AGCACAC706400.043.36950710
TCTGAAC698600.043.32193418
TCGGAAG711350.043.247043
GCACACG708100.043.2304311
CTGAACT700100.043.16805319
GTATGCC690950.043.0629745
TCTCGTA697350.042.5863341
AGATCTC693900.042.55161338
CGTATGC698900.042.54737544
ATCTCGT702350.042.23830840
AGTCACG710700.042.0372528
GTCACGA708550.041.8602629
AAGAGCA736800.041.732787
ATCGGAA742000.041.6086652
Produced by FastQC (version 0.11.2)