FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_1.3510000003836d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_1.3510000003836d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16150153
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3420732.1180790051958023TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG384550.043.35706731
CACACGT396200.043.3103912
ACGTCTG397600.043.13685615
CGTCTGA397800.043.12701816
ACACGTC397750.043.11333513
AGTCACT389850.043.08670828
TAGGCAT383300.043.05405835
GTATGCC388600.043.02100445
TCACTTA389650.042.88775330
TCTGAAC398150.042.88068418
TCTCGTA391650.042.7110741
CGTATGC393450.042.51928344
CTGAACT401400.042.46061719
AGCACAC405100.042.34220510
AGAGCAC405800.042.341248
GAGCACA406250.042.272199
GCACACG407000.042.19981811
GCATCTC394850.042.04009638
ATCTCGT399100.041.87431740
TCGGAAG412500.041.7920763
Produced by FastQC (version 0.11.2)