FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_input_0.35100000038337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_input_0.35100000038337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15989150
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC7963834.980771335561928TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT893900.043.9877412
ACACGTC893600.043.9798513
ACGTCTG896150.043.78605715
CGTCTGA895300.043.77498616
AGAGCAC903100.043.7190068
AGCACAC901850.043.64737710
GAGCACA904200.043.630989
TCTGAAC894600.043.6218418
TCGGAAG907100.043.6193283
GCACACG903900.043.54092411
GTATGCC881700.043.499645
CTGAACT895800.043.48051519
GTCACAT888900.043.3446529
CGTATGC891300.043.0563244
TCTCGTA891400.043.00834341
ATCACGA885450.042.90519334
ATCTCGT896050.042.68219440
AAGAGCA932800.042.384897
ATCGGAA938900.042.205822
CGGAAGA936150.042.1779444
Produced by FastQC (version 0.11.2)