FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_60.351000000382d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_60.351000000382d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16478588
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC15437759.368369425827018TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1749100.044.02750812
ACACGTC1750550.043.951213
CGTCTGA1748550.043.9287116
TCTGAAC1746600.043.86030618
ACGTCTG1752700.043.84895315
AGTCACC1717950.043.76633528
GTCTGAA1754850.043.70176717
GTATGCC1724550.043.7006445
AGAGCAC1767200.043.6618738
CTGAACT1751350.043.66040419
AGCACAC1769050.043.55262410
GAGCACA1771500.043.5479
ACTCCAG1737900.043.42550723
CTCCAGT1738600.043.41966624
CAGTCAC1743950.043.39249427
TCTCGTA1737700.043.37695741
AACTCCA1741200.043.3562622
CTCGTAT1742250.043.26133342
GCACACG1781450.043.25578311
AAGAGCA1786100.043.250247
Produced by FastQC (version 0.11.2)