FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_5.3510000003827b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_5.3510000003827b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17421129
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC193298211.095618429781446TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC2163650.044.02491418
CACACGT2177650.044.0104612
CGTCTGA2172300.044.00060716
ACACGTC2178350.043.99425513
ACGTCTG2180450.043.91972415
GTCTGAA2173600.043.88630317
CTGAACT2169200.043.8251419
ACAGTGA2117550.043.78367234
AGTCACA2145300.043.77652428
GTCACAC2149850.043.7584329
ACACAGT2131350.043.7489532
GTATGCC2145100.043.73889545
CAGTGAT2128350.043.69359235
CACAGTG2132700.043.67943233
ACTCCAG2154400.043.56871823
CTCCAGT2156500.043.5638524
AGTGATC2129400.043.5589836
CAGTCAC2164350.043.5553327
AGAGCAC2204850.043.5379378
AGCACAC2203200.043.5133610
Produced by FastQC (version 0.11.2)