FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_300.35100000038300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_300.35100000038300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15621084
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC9377956.003392594265545TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1064600.043.66425712
TCTGAAC1059200.043.58959218
CGTCTGA1063450.043.57111716
ACGTCTG1066300.043.48631315
AGTCACC1046800.043.4783728
TGTAATC1029250.043.47821836
ACACGTC1069800.043.45201513
CTTGTAA1030400.043.4460534
GTAATCT1032100.043.38023837
GTCTGAA1067500.043.32782717
GTATGCC1049050.043.2109145
CTGAACT1069950.043.0822419
AGAGCAC1082050.043.051588
CAGTCAC1060700.042.99543827
AGCACAC1083350.042.92930610
TAATCTC1045250.042.91197638
ACTCCAG1060850.042.89050323
CTCCAGT1061600.042.84324324
TCTCGTA1057200.042.82280341
GAGCACA1088350.042.8065039