FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_30.351000000382a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_30.351000000382a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16207812
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC15262149.416533212502712TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1731500.043.8667512
ACACGTC1731800.043.84876313
TCTGAAC1724250.043.82137318
ACGTCTG1729150.043.81739415
CGTCTGA1729050.043.80835716
GTCTGAA1732950.043.6487417
GTATGCC1706500.043.56915345
CTGAACT1735250.043.4748619
AGTCACG1709600.043.47457528
AGAGCAC1752100.043.4395988
CCAATAT1687750.043.41036635
AGCACAC1753350.043.39066710
GAGCACA1756250.043.3600129
GCCAATA1687950.043.33109734
CTCCAGT1717650.043.30293324
AATATCT1699850.043.30005637
ACTCCAG1719350.043.23525223
CAGTCAC1723450.043.23486327
AACTCCA1720750.043.22766522
TCTCGTA1719150.043.2223341
Produced by FastQC (version 0.11.2)