FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_1.35100000038252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_1.35100000038252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18046339
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC15812408.762109589097268TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT1782150.043.8963328
CGTCTGA1815200.043.86937316
TCTGAAC1812850.043.83933618
CACACGT1821550.043.7904512
ACGTCTG1822100.043.7488115
ACACGTC1823700.043.74252313
GTCTGAA1822200.043.67738717
CTGAACT1818650.043.61910619
CAGTCAC1800100.043.4286127
CTCCAGT1793500.043.411524
ACTCCAG1795500.043.3994823
ACCAATC1735000.043.3644336
ACTGACC1779000.043.32520332
AACTCCA1800800.043.31060422
GTCACTG1813900.043.26701729
GACCAAT1741500.043.263335
TCACTGA1810800.043.20179430
GTATGCC1783850.043.1697545
AGCACAC1849800.043.1241210
CCAATCT1750400.043.1053937
Produced by FastQC (version 0.11.2)