FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l03n01_tga1_rep2_chip_0.3510000003821e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l03n01_tga1_rep2_chip_0.3510000003821e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17796985
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC8247054.633959066662134TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC946700.043.3135718
AGTCACC937100.043.28986428
CGTCTGA952900.043.2050316
CACACGT949150.043.20487212
GTATGCC938700.043.1710645
GATGTAT933700.043.1587535
ACACGTC952200.043.1326413
ACGTCTG956950.043.06437715
GTCTGAA957650.042.95313617
TCTCGTA944600.042.8921541
CTGAACT954150.042.79144319
CTCCAGT941300.042.71572524
ATGTATC943300.042.69566336
CTCGTAT949500.042.68204542
CAGTCAC948200.042.66683227
GTATCTC949900.042.64335638
TCGTATG954000.042.55383343
TATCTCG953550.042.5438439
ACTCCAG944900.042.5291723
CGTATGC956450.042.40751644