FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_9.35100000038387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_9.35100000038387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043017
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3834282.2497659892024986TruSeq Adapter, Index 9 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG1034480.6069817333398189No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG850680.49913697791887435No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA396490.23264073491213438No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC393870.23110344840939842No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT383930.22527114770817866No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG378240.2219325369446032No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC264280.1550664415813233No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT239900.14076146259784872No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG222790.1307221602841797No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC201750.11837692821640676No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT197670.11598298587626828No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC192380.11287907534211813No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA182550.10711131720399034No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC177230.1039898041526333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG434000.043.856811
AGCACAC438400.043.33452210
GTATGCC432200.043.3088945
CGTATGC433250.043.2405444
ACGTCTG442850.043.03775815
GTCACGA434300.042.90059329
AGTCACG436000.042.86069528
CACACGT444700.042.8369712
ACACGTC444650.042.83166513
TCGTATG438750.042.75015343
CTCGTAT438350.042.68650442
CACGTCT448100.042.53352414
GAGCACA449350.042.368659
CGTCTGA449000.042.36821416
AGAGCAC452100.042.150748
TCTCGTA446300.041.96203241
CAGTCAC445950.041.95484527
CTGAACT453400.041.83795519
TCACGAT446350.041.7758530
CCAGTCA450300.041.63948426