FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_7.3510000003831d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_7.3510000003831d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14558888
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC3005372.0642854042149374TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG843770.5795566254785393No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG682600.46885448943628116No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG305200.2096313949252168No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA304220.20895826659288816No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC301920.20737847560885142No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT280120.19240480454276454No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC207460.14249714676010972No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT194600.1336640545624089No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG189720.13031215021367015No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT175030.12022209388519232No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC159460.10952759578890915No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC152200.10454095120451506No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG146200.100419757333115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG339550.043.53995511
GTATGCC334650.043.16465845
CGTATGC336200.043.0191944
AGCACAC343750.042.9621610
ACGTCTG346750.042.727615
ACACGTC347700.042.56468613
CACACGT347500.042.5438612
CTCGTAT340350.042.5084542
TCGTATG341550.042.3656943
GAGCACA350950.042.1512839
CACGTCT351800.042.08867614
CGTCTGA352600.041.98679716
CAGTCAC344850.041.89853327
AGAGCAC353500.041.7581148
CATCTCG349700.041.61739339
TCTCGTA348850.041.4606641
CTGAACT355400.041.3901219
CCAGTCA350550.041.33278326
AGTCACC350150.041.18723728
TCCAGTC356550.040.97167225
Produced by FastQC (version 0.11.2)