FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_6.351000000382e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_6.351000000382e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591584
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1294580.7359087163498182TruSeq Adapter, Index 6 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG1154130.6560694022778164No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG957440.5442602553584714No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA440210.2502389779112557No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC414310.23551602857366344No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG412310.23437912128890723No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT384190.21839420486523556No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC278280.15818927960097284No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT267310.15195334314408526No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG254820.144853357150783No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT229250.13031799751517542No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC215280.12237670013115363No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC206410.11733451632326003No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC202840.11530513681997027No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG199700.1135201923829031No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA183380.10424302893929278No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT182280.1036177299326769No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCG178220.10130980814462187No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC176630.10040596685324073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG155450.041.639311
GTATGCC154600.040.80994845
CGTATGC155600.040.57682844
TCACGCC156100.040.37547330
ACGTCTG161200.039.9731615
CACGCCA157050.039.88767231
AGCACAC162650.039.8237310
GTCACGC160400.039.5982229
CTCGTAT159300.039.53616342
CACACGT164150.039.39129312
AGTCACG159850.039.35297828
ACACGTC164700.039.205113
TCGTATG162750.038.822543
CGTCTGA167000.038.7061216
TATCTCG164800.038.55858239
CACGTCT169450.038.1199314
AGAGCAC170900.038.0066038
CGCCAAT164950.037.96443633
TCTCGTA167850.037.6299341
GAGCACA173300.037.5711339
Produced by FastQC (version 0.11.2)