FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_5.351000000382be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_5.351000000382be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159744
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG1151010.6338250142733289No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG923050.5082946103204979No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC893850.4922150884946396TruSeq Adapter, Index 5 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA444860.24497041367984043No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC406060.22360447371945333No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG384660.2118201666278996No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT348510.19191349834006471No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC275030.15145037286869242No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT261200.14383462674363692No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG247450.13626293410303583No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT233740.12871326820466192No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC211220.11631221233074651No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC197960.10901034728242864No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC194370.10703344716753715No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA188030.10354220852452545No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG181780.10010053005152496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG109600.039.8236711
CGTATGC110000.039.13179444
GTATGCC110100.038.9325445
AGCACAC114900.037.9279810
ACGTCTG115600.037.8162315
ACACGTC116550.037.27520813
CACACGT116750.037.2691712
CTCGTAT117250.036.6355742
TCGTATG117450.036.611543
CACGTCT119900.036.32866314
CGTCTGA120200.036.2567116
ACACAGT118950.035.56330532
AGAGCAC124750.035.185758
CACAGTG121650.034.97812333
CACACAG121600.034.89930731
GAGCACA126350.034.6689579
TCTCGTA124750.034.55986441
GTCACAC124800.034.19967329
CAGTCAC125700.034.13210727
CTGAACT130700.033.2235619
Produced by FastQC (version 0.11.2)