FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_4.35100000038288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_4.35100000038288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17414616
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG995180.5714625002354344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC859020.4932753039171234TruSeq Adapter, Index 4 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG787050.45194794992895626No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA373570.21451520952285139No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG358390.20579839371709374No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC350430.2012275206068282No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT279270.16036529315375084No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC223450.12831175835286865No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT223040.12807632393387255No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG208810.11990502690383757No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT195990.11254339458303302No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC188560.10827686352659169No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC178020.10222447626752149No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC176330.10125402707702542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG110050.039.70236611
GTATGCC109300.039.07177745
CGTATGC110100.038.93104644
ACTGACC112250.037.94548832
AGCACAC116050.037.7078410
ACGTCTG117100.037.50626815
CACACGT117100.037.4273712
CTCGTAT115300.037.0782242
TCGTATG116750.036.6755443
ACACGTC119400.036.64987613
CGTCTGA121600.036.0812816
CACGTCT121250.036.0739914
AGAGCAC125500.034.993988
GAGCACA126050.034.912689
CAGTCAC124250.034.4765727
AGTCACT124750.034.39248728
TCTCGTA126000.034.0907341
CACTGAC127300.033.742231
CCAGTCA127900.033.59822526
CTGACCA126600.033.502633
Produced by FastQC (version 0.11.2)