FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_3.3510000003826e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_3.3510000003826e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17343761
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1742901.0049146779640241TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG925940.5338749767135282No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG737620.42529414467830823No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA364260.2100236505795946No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG329990.19026438383231872No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC311220.17944204835387204No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT263240.15177792175526403No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT213640.12317974169501068No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC209570.12083307651667939No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT195360.11263992855990115No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG190500.10983776817496504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG200500.042.47260311
GTATGCC199450.041.93203745
CGTATGC201100.041.67749844
TAGGCAT200500.041.36598635
AGCACAC205900.041.3149910
ACGTCTG207550.040.92304215
CACTTAG203500.040.91026331
ACTTAGG203400.040.8861232
CTCGTAT205000.040.87445442
ACACGTC208150.040.8359713
CACACGT208400.040.83017312
TCGTATG206950.040.48931543
TTAGGCA207150.040.1248234
CACGTCT213050.039.92983614
GAGCACA216100.039.5106669
AGAGCAC216700.039.4531828
AGGCATC210700.039.40617436
CGTCTGA216300.039.26757416
CAGTCAC212000.039.21341727
TCACTTA213300.038.87241730
Produced by FastQC (version 0.11.2)