FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_12.3510000003841c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_12.3510000003841c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15347319
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC3477102.265607432803084TruSeq Adapter, Index 12 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG987450.6434022776225606No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG771460.5026675994680243No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT375190.2444661507329065No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG367290.23931867188008538No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA357140.23270513892361266No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC354330.23087420024305222No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC227120.1479867591205995No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG215480.14040237255770863No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT206740.13470756683952423No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT184620.12029462605162504No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC182930.1191934565248823No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC182100.11865264545553526No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA174090.11343349284653562No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC166150.10825995080964955No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG165150.10760837120802663No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT160330.10446775752820411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG395750.043.79128311
GTATGCC392400.043.37256645
CGTATGC394300.043.1922444
AGCACAC400500.043.18203410
CACACGT402850.042.9915712
ACGTCTG403450.042.9414415
ACACGTC403400.042.92179513
CACGTCT405850.042.65978214
CTCGTAT398600.042.6592542
TCGTATG399800.042.63251543
CGTCTGA406300.042.48530616
GAGCACA408700.042.337669
AGAGCAC409750.042.21278
CAGTCAC403100.042.04384627
AGTCACC404250.041.9298128
CTGAACT411200.041.9189919
TCTCGTA406450.041.89733541
TCCAGTC411250.041.5662425
CCAGTCA409750.041.37248226
TCGGAAG418700.041.221583