FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l02n01_hho5_pchx_11.351000000383ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l02n01_hho5_pchx_11.351000000383ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622836
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2801991.7935219956223059TruSeq Adapter, Index 11 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG1013620.6488066571267854No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG806990.5165451394356313No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT370560.23719125003936545No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG369830.23672398532507158No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC363750.23283224633478838No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA344820.22071536819563364No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC232300.14869259332940576No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG219470.14048025595352853No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT211370.13529553789081572No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC191760.12274339946985297No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT189890.12154643369488101No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC182980.11712342112533217No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG168260.10770131620148865No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC166350.10647874688052797No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA161730.1035215373188325No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT161420.10332310983742005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG332100.043.4858111
AGCACAC337300.042.77538710
ACGTCTG336600.042.73325715
ACACGTC338350.042.60939413
GTATGCC326700.042.53551545
CACACGT340000.042.435712
CGTATGC328250.042.3553644
CGGCTAC330700.042.22022633
CACGTCT341950.042.10415314
CACGGCT333550.042.10166531
ACGGCTA332500.042.0857332
CTCGTAT331400.042.00096542
AGTCACG334900.041.933328
GGCTACA327650.041.90588434
AGAGCAC344750.041.8705988
GAGCACA345150.041.8546649
CGTCTGA343700.041.8177616
TCGTATG333500.041.79721543
GCTACAT331400.041.39774335
CATCTCG333100.041.3215739