FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_90_2.35100000038394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_90_2.35100000038394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17272940
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2196751.2717869685183876TruSeq Adapter, Index 11 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270200.1564296523927021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG251350.042.81451828
TCGTATG254700.042.00073643
CTCGTAT254350.041.934742
ACGTCTG261800.041.91587415
CACGTCT263300.041.68538714
CGTCTGA265400.041.31340816
CACACGT265750.041.26483512
GTCACGG264300.040.94698729
GTATGCC260650.040.9363745
ACACGTC269950.040.6894813
ATCGGAA270950.040.556412
CAGTCAC267150.040.26550727
ACGGCTA267050.040.26148632
TCTCGTA267400.039.95800441
CACGGCT272500.039.62138431
GATCGGA279900.039.5534971
GTCTGAA280350.039.11834317
GCTACAT272550.038.8741235
CCAGTCA277650.038.7022626
TCGGAAG284300.038.6155243
Produced by FastQC (version 0.11.2)