FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_45_3.35100000038245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_45_3.35100000038245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18331064
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC395100.21553577031862414TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG52300.034.92489228
TCGTATG55400.033.42245543
CTCGTAT56700.032.57679442
CGTCTGA60050.031.01907716
ACGTCTG61550.030.33622715
GTCACGA60650.030.19152329
CACACGT64150.029.20959312
GTATGCC63950.029.16390245
CACGTCT65300.028.7659614
CGTATGC67100.027.76120444
ATCGGAA67500.027.666072
TCTCGTA69250.026.96766341
ACACGTC73200.025.7519513
TCACGAT71750.025.61902830
CAGTCAC72800.025.24456427
TCGGAAG77050.024.3217373
GTCTGAA78350.023.91759517
GATCGGA79300.023.6425251
CCAGTCA80650.023.26156226
GCACACG85750.022.14040211
Produced by FastQC (version 0.11.2)