FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_45_2.3510000003822b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_45_2.3510000003822b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19619945
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC560410.28563280885853654TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT75350.035.08320642
ACGTCTG77350.034.9293815
TCGTATG76650.034.5175443
CGTCTGA78750.034.19414516
CACGTCT83600.032.5060714
GTATGCC84000.031.84381945
CACACGT86350.031.28736112
ATCGGAA88000.030.6291852
GTCACAC88150.030.29183229
CGTATGC87500.030.28724944
TCTCGTA90900.029.42963441
AATCTCG89400.029.14324439
ACACGTC93400.029.02207213
AGTCACA91300.028.95052528
CACACTT93550.028.33120531
CAGTCAC94900.028.06556327
TCACACT95650.027.87384630
GTCTGAA98500.027.38363817
TCGGAAG98800.027.3469333
GATCGGA99450.027.2972981
Produced by FastQC (version 0.11.2)