FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_45_1.351000000381f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_45_1.351000000381f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21614491
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC485890.2247982614996578TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA76450.033.95758416
TCGTATG75350.033.888843
CTCGTAT75800.033.56889342
ACGTCTG78500.033.27139715
CACGTCT81950.031.78813714
GTATGCC85450.029.82890945
CACACGT88600.029.57880212
CGTATGC87050.029.25493644
ATCGGAA89100.029.014972
AGTCACC91000.028.05723428
ACACGTC93700.027.89683313
TCTCGTA93000.027.65202941
CAGTCAC93400.027.36029827
GTCTGAA99950.026.28866417
CCAGTCA98700.026.11901926
TCGGAAG100250.025.7851833
GATCGGA103000.025.301871
GCACACG116200.023.03722811
AGCACAC117800.022.55246210
TCCAGTC117250.022.33209825
Produced by FastQC (version 0.11.2)