FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_20_3.3510000003837a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_20_3.3510000003837a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236550
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3130701.6274747810808072TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559000.2905926478500563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255530.13283566959771892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG353100.042.52063832
TCGTATG354650.042.42665543
CACTTAG354550.042.3150131
CTTAGGC355550.042.22840533
CTCGTAT356200.042.1851942
TTAGGCA354800.042.06400734
ACGTCTG363050.042.01218415
CACGTCT363750.041.974314
CGTATGC358550.041.96893744
CGTCTGA363150.041.95725316
TCACTTA358650.041.83127630
GTATGCC362700.041.45760745
CACACGT368000.041.44405712
ACACGTC369000.041.35612513
TAGGCAT360950.041.3292535
ATCGGAA369600.041.2794232
AGTCACT366250.041.03435528
GTCACTT368900.040.95407529
TCTCGTA370050.040.71209741
TCGGAAG378000.040.3629153
Produced by FastQC (version 0.11.2)