FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_20_2.35100000038351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_20_2.35100000038351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18101267
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC6289313.4745136901190397TruSeq Adapter, Index 2 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1541790.8517580564940566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA696830.3849620029360376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA705450.043.4465072
ACGTCTG703350.043.2428815
GATCGGA717400.043.087871
CACGTCT706700.043.07255614
CGTCTGA706300.042.94760516
CACACGT707900.042.94738812
ACACGTC709850.042.8579313
CTCGTAT693600.042.78314642
TATCTCG695000.042.78037339
TCGGAAG716300.042.7232933
TCGTATG696400.042.66927743
GTATGCC696550.042.66155245
AGTCACC703800.042.4245528
GTATCTC703500.042.2986638
CGATGTA702050.042.2834734
TCTCGTA704500.042.1650541
CCGATGT708500.042.14940333
CAGTCAC710150.042.05423727
ACCGATG711500.041.91044232
CACCGAT711400.041.9100131