FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_n_20_1.3510000003832a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_n_20_1.3510000003832a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16455969
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514160.3124458972911288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277380.1685588979901457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC232400.14122535111727544TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39850.027.15591443
CTCGTAT39100.026.9863342
ACGTCTG42850.026.0922215
CACGTCT43000.025.94872914
CGTATGC42000.025.4432344
CGTCTGA43900.025.3656616
CACACGT45800.024.45938712
GTATGCC45650.023.4583145
ACACGTC48850.022.88619213
TCTCGTA50300.021.38123141
ATCGGAA52100.021.3766162
GTCACAT52000.020.41895929
TCACGAT53650.020.04706235
TCGGAAG57700.019.4556033
AGTCACA56050.019.38449928
ATCACGA56800.018.65760434
CAGTCAC59150.018.48260927
GATCGGA60750.018.4492071
GTCTGAA62800.017.83919117
GCACACG64500.017.71681811
Produced by FastQC (version 0.11.2)