FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_k_45_3.351000000382f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_k_45_3.351000000382f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18602170
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC240900.12950102057985707TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG38350.032.14400528
CTCGTAT42050.029.15907142
TCGTATG42600.028.78260243
TATCTCG46500.026.66045239
ACGTCTG49150.025.9063915
CGTCTGA49550.025.28864116
CGTATGC49500.024.95094344
CACGTCT51100.024.74149114
GTATGCC52150.024.0280445
CACACGT52900.023.77057812
ATCGGAA53500.023.382442
CGCCAAT54000.022.37465533
GTCACGC56600.022.01860429
ACACGTC58850.021.63483613
TCTCGTA58200.021.53282741
GCCAATA56550.021.28614634
TCGGAAG61700.020.5649553
CAGTCAC62050.020.1565727
TCACGCC64400.019.49472830
ATATCTC65900.018.81200238
Produced by FastQC (version 0.11.2)