FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_k_45_2.351000000382cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_k_45_2.351000000382cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18443499
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC813870.44127743873329023TruSeq Adapter, Index 5 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337920.1832190301851075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG104550.038.06612443
CTCGTAT105250.037.8129542
ACGTCTG109300.037.3570415
CACGTCT112250.036.37507614
CGTCTGA113200.035.9308916
CACACGT115500.035.32937612
GTATGCC114250.034.93157245
ATCGGAA118100.034.6727372
ACACGTC119650.034.02879313
GTCACAC117750.033.9103529
ACACAGT118250.033.48705732
AGTCACA118950.033.2835828
CAGTCAC123300.032.36475827
CACAGTG122500.032.3627833
TCTCGTA123800.032.27703541
TCGGAAG128800.031.8937533
GTCTGAA128450.031.85771217
AGTGATC126750.031.17189636
GATCGGA131900.031.141371
CGTATGC128150.031.01977544
Produced by FastQC (version 0.11.2)