FastQCFastQC Report
Tue 27 Oct 2015
C7KCWACXX_l01n01_k_45_1.35100000038295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l01n01_k_45_1.35100000038295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17848637
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC641190.35923751488699107TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT86300.037.12256642
TCGTATG88400.036.49519343
CGTCTGA91050.036.02380816
ACGTCTG92250.035.82345215
CACGTCT93600.035.21003714
CACACGT95200.034.5931912
ACTGACC92850.034.43284232
AGTCACT94750.034.0928228
CGTATGC94450.033.96531744
ATCGGAA97500.033.8529972
GTATGCC97950.032.9583645
ACACGTC101500.032.60116213
AATCTCG99300.031.90225839
TCTCGTA100450.031.89531141
TCGGAAG103750.031.8322263
CAGTCAC106050.030.46002427
GATCGGA109300.030.286841
CACTGAC107550.030.20768731
GACCAAT104800.030.1004535
GTCTGAA110000.030.04284917
Produced by FastQC (version 0.11.2)