FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l08n01.3310000000ecc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l08n01.3310000000ecc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58485243
Sequences flagged as poor quality0
Sequence length51
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG16096450.056.2080846
GTCCACG28157750.056.096256
GGTGTCG5202100.055.832395
AGTGTCG4405950.055.7167785
CGTCCAC7432000.055.6920785
GGTCCAC7068350.055.576275
AGTCCAC8087250.055.5615775
CCGTCCA4043000.055.474644
TGTGTCG5164100.055.467575
CAGTGTC2387300.055.3644454
TTGAGTG551900.055.210242
CAGTCCA4846550.055.166044
ATATGGT1176800.055.1626741
TGTCCAC6399700.055.0996485
TGTCGGT16362650.054.978517
TCCACGA28824700.054.978327
CGGTCCA1762350.054.9567644
ATCAAGT378750.054.8630681
TACCGTC2749600.054.840492
CACGTGT383450.054.701183
Produced by FastQC (version 0.11.2)