FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 tcfgrna-18h-2.3410000000d751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 tcfgrna-18h-2.3410000000d751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25019048
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG264250.017.6000582
TAACTTT268950.017.1925811
TTTGTGT575350.015.36521152
CTTTGTG611500.014.5744891
CGCGTAA16800.013.1383411
TTGTGTT742050.012.5502063
TGTGTTT790700.011.8093224
GCGCGAT18600.011.7457981
GTGTTTG803100.011.620945
GCGTAAC32050.011.5952371
ACTTTGT435550.011.4695731
CACGGTA88550.011.25824445
CGCGATT31050.010.5179261
TGTTTGA925250.010.1177686
CTCACGG102000.09.88381643
TCACGGT102200.09.86465144
CGGTACT57650.09.64168945
TGACGGG126800.09.45701410
CCTCACG110700.09.16780342
GTTTGAC495050.09.130137
Produced by FastQC (version 0.11.2)