FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 tcfgrna-18h-1.3410000000d5d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 tcfgrna-18h-1.3410000000d5d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25893314
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG279900.017.0425642
TAACTTT283400.016.8157881
TTTGTGT638250.016.6822992
CTTTGTG664750.016.0929871
TTGTGTT820250.013.5102063
CGCGTAA18500.013.2695491
TGTGTTT884550.012.5790184
GTGTTTG900500.012.3354625
GCGTAAC30350.012.0956811
ACTTTGT453150.011.4857491
GCGCGAT20100.011.4289291
CACGGTA98650.011.06324945
TGTTTGA1041250.010.69757656
CGCGATT29500.010.688271
CTACCCG69150.010.50911618
GAGGCTA68750.010.27551514
CTAAGCG72300.010.05293843
CTCACGG110300.010.01693443
GCTACCC73300.010.00618717
TCACGGT109900.09.99217844
Produced by FastQC (version 0.11.2)