FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 fzgrna-18h-2.3410000000d7d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 fzgrna-18h-2.3410000000d7d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26477323
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG264150.016.5504762
TAACTTT286550.015.4681391
TTTGTGT545850.015.3464552
CGCGTAA20600.015.306441
GCGTAAC33650.014.1224741
CTTTGTG607550.013.9015311
TTGTGTT703050.012.5295043
TGTGTTT748150.011.8133194
GTGTTTG766350.011.5529685
GCGCGAT18450.011.2306491
CGCGATT29850.010.7895941
ACTTTGT439000.010.5839581
CTAAGCG74700.010.12178343
GAGGCTA72950.010.08493314
CTACCCG75250.010.07572818
TGTTTGA890850.09.9555086
AGGCTAC75950.09.74586515
GCTACCC78250.09.68943817
TAAGCGG85250.09.08041544
TACTAAG84200.08.97923441
Produced by FastQC (version 0.11.2)