FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 fzgrna-18h-1.3410000000d638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 fzgrna-18h-1.3410000000d638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24021907
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG239100.017.8608232
TAACTTT249600.017.2254771
TTTGTGT524150.015.6296742
CTTTGTG566850.014.5459311
CGCGTAA14050.012.8240021
TTGTGTT669050.012.8163753
CGCGATT31200.012.4882461
GCGCGAT19500.012.2428111
TGTGTTT709750.012.1416894
GTGTTTG730850.011.7691125
ACTTTGT400400.011.2723271
GCGTAAC23500.010.8298021
CACGGTA95500.010.74532545
TGTTTGA846650.010.2201386
CTACCCG67000.010.07396718
GAGGCTA66050.09.94625614
CTAAGCG69950.09.58680643
TCACGGT109200.09.54143544
AGGCTAC69250.09.48666615
GCTACCC70850.09.46303717
Produced by FastQC (version 0.11.2)