FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 controlgrna-18h-2.3410000000d6d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 controlgrna-18h-2.3410000000d6d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22805868
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG255900.017.6204432
TAACTTT258400.017.3718191
TTTGTGT538550.015.1241842
CTTTGTG578100.014.0960051
TTGTGTT694550.012.3333
CGCGTAA14450.012.3137331
TGTGTTT739250.011.6177014
GTGTTTG750800.011.4085115
ACTTTGT416050.011.1736391
GCGTAAC24150.011.0051351
CACGGTA84300.010.54418945
GCGCGAT19250.010.4133341
CGCGATT29150.010.1219271
AGGCTAC64100.09.96801715
GAGGCTA64100.09.93291814
TGTTTGA879750.09.7690536
CTACCCG65950.09.7566918
CTAAGCG67450.09.50827743
GCTACCC68500.09.49201817
CGGTACT56450.09.44774245
Produced by FastQC (version 0.11.2)