FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l07n01 controlgrna-18h-1.3410000000d57c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l07n01 controlgrna-18h-1.3410000000d57c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27477835
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT303850.019.8274731
AACTTTG321100.018.5970922
TTTGTGT800550.017.6841722
CTTTGTG835400.016.9843461
TTGTGTT1019250.014.4636413
TGTGTTT1102000.013.3898074
GTGTTTG1124600.013.1202475
CACGGTA163700.012.81152245
ACTTTGT546100.011.8403291
TGTTTGA1281250.011.5437136
CTCACGG185150.011.375813543
TCACGGT189800.011.15653444
CTACCCG84650.010.737699518
GAGGCTA83050.010.72766714
CGCGATT34750.010.6937961
CCTCACG202500.010.43434942
GTTTGAC668850.010.3199217
AGGCTAC87000.010.29238215
GCTACCC89600.010.21981917
CCCTCAC210750.010.15364441
Produced by FastQC (version 0.11.2)