FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 tcfgrna-15h-2.3410000000d6e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 tcfgrna-15h-2.3410000000d6e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28874484
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG333550.020.020192
TAACTTT348700.019.1060621
TTTGTGT796900.015.8558122
CTTTGTG846350.015.0436751
CGCGATT32750.013.4106011
TTGTGTT1019050.013.0241123
ACTTTGT585150.012.7173841
GCGTAAC31400.012.624321
TGTGTTT1079300.012.345014
CGCGTAA18800.012.219891
GTGTTTG1113400.011.9871285
GCGCGAT21150.011.9270381
ATCCGGG15900.011.03920745
CACGGTA135550.010.9900345
GAGGCTA93000.010.789514
CTAAGCG94850.010.74714343
CTACCCG97300.010.54406718
AGGCTAC95450.010.5127215
TGTTTGA1280950.010.4222936
CGATTCG66650.010.26215652
Produced by FastQC (version 0.11.2)