FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 tcfgrna-15h-1.3410000000d5e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 tcfgrna-15h-1.3410000000d5e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27642540
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG324900.018.4272442
TAACTTT328750.018.1954941
TTTGTGT734400.016.3320712
CTTTGTG790200.015.2054361
TTGTGTT926400.013.5859243
GCGCGAT20600.012.9007881
CGCGTAA15900.012.8897881
TGTGTTT993200.012.7220084
GTGTTTG1025650.012.3349035
ACTTTGT541250.012.291761
GCGTAAC29250.012.0885781
CGCGATT27700.011.6267341
CAGACCC20500.011.41587545
GAGGCTA86450.011.11267814
CTACCCG89450.011.09226818
CTAAGCG90100.011.01388143
AGGCTAC88200.010.96887315
TGACGGG138950.010.84855910
GCTACCC91750.010.81420617
TGTTTGA1171750.010.8062896
Produced by FastQC (version 0.11.2)