FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 fzgrna-15h-2.3410000000d76d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 fzgrna-15h-2.3410000000d76d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27879371
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG326250.018.8955522
TAACTTT340450.018.1457391
TTTGTGT793250.017.2474332
CTTTGTG849800.016.2168271
TTGTGTT986350.014.4753333
TGTGTTT1050600.013.6436244
GTGTTTG1089750.013.1410815
CGCGTAA17650.012.76021
GCGTAAC30450.012.573721
CGCGATT29300.012.3754331
ACTTTGT564400.012.1706851
TGTTTGA1237850.011.5686366
CACGGTA122850.011.2464845
GCGCGAT19450.011.2319281
TGACGGG136750.011.0558710
CTAAGCG86550.010.42551743
GTTTGAC639100.010.3356787
GACGGGC118550.010.30502911
TTGACGG156350.010.2742779
CTACCCG87800.010.12186518
Produced by FastQC (version 0.11.2)