FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 fzgrna-15h-1.3410000000d62b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 fzgrna-15h-1.3410000000d62b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25174825
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG291200.018.8830932
TAACTTT311250.017.7279131
TTTGTGT644650.015.5275092
CTTTGTG695350.014.4940731
GCGCGAT17050.013.4733831
CGCGTAA14100.013.2574451
GCGTAAC27150.013.1894951
TTGTGTT822200.012.8065383
ACTTTGT487750.012.4440681
TGTGTTT874700.012.1201924
GTGTTTG895000.011.8326945
CGCGATT25400.011.5268471
CACGGTA113300.011.00335545
TGTTTGA1038150.010.2311266
CAGACCC19550.010.12934245
CGGTACT73550.010.00484545
TCACGGT127050.09.88322744
CTAAGCG89550.09.82540443
CTACCCG89000.09.7831218
GAGGCTA86800.09.77178114
Produced by FastQC (version 0.11.2)