FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 controlgrna-15h-2.3410000000d66e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 controlgrna-15h-2.3410000000d66e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26945646
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCG286090.10617299729982352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG368200.022.9143332
TAACTTT383800.021.9404141
TTTGTGT1004750.016.857042
CTTTGTG1022800.016.606961
ACTTTGT659450.015.3921771
GCGTAAC31950.014.943741
CGCGTAA20300.014.4225351
TTGTGTT1235000.014.3664793
CGCGATT28750.014.3353251
TGTGTTT1316000.013.5488964
GCGCGAT19450.013.200171
GTGTTTG1370950.013.0074995
CACGGTA131000.012.50524845
CTAAGCG103700.011.58746143
TGTTTGA1567050.011.36652856
GAGGCTA106150.011.16965814
ATCCGGG14600.011.09716145
AGGCTAC107700.011.02989815
TAAGCGG112200.010.95028944
CTACCCG109650.010.936336518
Produced by FastQC (version 0.11.2)