FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l06n01 controlgrna-15h-1.3410000000d589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l06n01 controlgrna-15h-1.3410000000d589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31453937
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTG365300.018.6004032
TAACTTT372600.018.2726331
TTTGTGT827750.015.387182
CGCGTAA19450.014.937431
CGCGATT35800.014.5323131
CTTTGTG895300.014.3463221
GCGTAAC32300.012.8298351
TTGTGTT1041800.012.8282433
ACTTTGT628900.012.2010251
TGTGTTT1107600.012.1027114
GCGCGAT24250.011.7949951
CACGGTA126750.011.770555545
GTGTTTG1143300.011.7425485
CTCACGG146150.010.36193243
TCACGGT145450.010.33444644
TGTTTGA1320100.010.2155536
CTAAGCG95400.010.16607743
GAGGCTA91600.010.14390614
CTACCCG96650.09.93991918
AGGCTAC93300.09.93510315
Produced by FastQC (version 0.11.2)