FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8441b-062415.3410000000d893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8441b-062415.3410000000d893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421264
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC667604.697227256864312TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA427383.0070416192909972No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC351782.475120737596956No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC79100.5565468484391358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA77000.044.0194321
CACACGT76150.043.6363712
GCACACG76050.043.63458311
ACGTCTG77250.043.39361615
TCGGAAG78250.043.3278273
CCAGGGA48500.043.325465
AGCACAC76750.043.2952410
GAGCACA77250.043.2188769
ACACGTC77400.043.16418513
CACGTCT77450.043.1072714
AGTCACG76800.043.0958428
AGAGCAC77600.043.081938
GTCACGC76950.042.9533529
ATCGGAA79050.042.9462662
TCACGCC77050.042.86840430
CCAGTCA77000.042.77935426
TATAAGC49250.042.75704214
CAGGGAT49200.042.754776
GTATGCC77050.042.725445
AGGGATT49300.042.713687
Produced by FastQC (version 0.11.2)