FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8441a-062415.3410000000d850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8441a-062415.3410000000d850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5767490
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1386622.404200093975022TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG164500.043.3982835
GCACACG173100.043.00874311
GTCCCGA161850.042.99674636
ACCCGTC171750.042.72098532
GATCGGA177600.042.6048551
TCACCCG172450.042.53452730
CCCGATC162400.042.53395538
ACACGTC175450.042.5096213
CCCGTCC171900.042.4593533
ACGTCTG175250.042.45506315
CACGTCT175050.042.4521614
CACACGT175500.042.44623612
CGTCTGA175600.042.37301316
CACCCGT173500.042.27711531
CCGTCCC172550.042.18205334
TCCCGAT164300.042.1779337
AGCACAC177950.042.13998810
GAGCACA178700.042.04989
CCGATCT164600.041.9129639
GAACTCC176950.041.85755521