FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8440d-062415.3410000000d645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8440d-062415.3410000000d645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586206
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC115943032.33026769795154TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1305413.6400864869446985No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC986562.7509853031309413No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC251120.7002386365981207No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG47190.1315875328968832TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1268250.044.9350741
TCGGAAG1274300.044.6339343
ATCGGAA1276500.044.611652
CGGAAGA1276050.044.5744864
GAGCACA1270650.044.517919
GCACACG1263800.044.51068511
AGAGCAC1272000.044.5060428
AGCACAC1268450.044.48932610
CACACGT1266650.044.4318512
ACACGTC1267400.044.41620613
GGAAGAG1282450.044.3608135
GAAGAGC1279450.044.3522956
CACGTCT1266900.044.34663414
ACGTCTG1267650.044.306215
AAGAGCA1282000.044.253647
CGTCTGA1269550.044.22101216
GTCTGAA1271300.044.17899317
TCTGAAC1271900.044.11385318
GAACTCC1267900.044.05607221
TGAACTC1272600.044.03301620
Produced by FastQC (version 0.11.2)