FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8440c-062415.3410000000d5f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8440c-062415.3410000000d5f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658246
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC3195448.734896450375398TruSeq Adapter, Index 12 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA78970.21586847904706244No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC71080.19430076599550714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA346400.044.3480151
GCACACG345000.044.10555611
ACACGTC345600.044.0615413
CACACGT345900.044.02982712
CACGTCT345600.044.02187314
ACGTCTG345550.044.0087115
TCGGAAG349100.043.9589653
GAGCACA348500.043.853899
CGTCTGA346550.043.82388716
AGCACAC348100.043.80326510
ATCGGAA350800.043.7715952
GAACTCC346050.043.72877521
AGAGCAC350050.043.653288
GTATGCC344350.043.5853345
CCAGTCA346250.043.5747526
CAGTCAC347100.043.5717627
TCCAGTC346750.043.5431725
GTCTGAA349050.043.53341317
TCTGAAC349500.043.41298318
AGTCACC347800.043.40643728
Produced by FastQC (version 0.11.2)